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KMID : 0381120230450070855
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Genes and Genomics
2023 Volume.45 No. 7 p.855 ~ p.866
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Inactivation of epithelial sodium ion channel molecules serves as effective diagnostic biomarkers in clear cell renal cell carcinoma
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Qian Zheng
Pablo Bellosta-Lopez
Ran Zhao
Peipei Han
Jun Zhao
Limei Li
Xiaohui Zhou
Ping Li
Yingxi Mo
Xinli Pan
Wenqi Luo
Xiaoying Zhou
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Abstract
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Background : Non-voltage-gated sodium channel, also known as the epithelial sodium channel (ENaC), formed by heteromeric complexes consisting of SCNN1A, SCNN1B, and SCNN1G, is responsible for maintaining sodium ion and body fluid homeostasis in epithelial cells. However, no systematic study of SCNN1 family members has been conducted in renal clear cell carcinoma (ccRCC) to date.
Objective : To investigate the abnormal expression of SCNN1 family in ccRCC and its potential correlation with clinical parameters.
Methods : The transcription and protein expression levels of SCNN1 family members in ccRCC were analyzed based on the TCGA database, and were confirmed by quantitative RT-PCR and immunohistochemical staining assays, respectively. The area under curve (AUC) was used to evaluate the diagnostic value of SCNN1 family members for ccRCC patients.
Results : The mRNA and protein expression of SCNN1 family members was significantly downregulated in ccRCC compared with normal kidney tissues, which might be due to DNA hypermethylation in the promoter region. It is worth noting that the AUC of SCNN1A, SCNN1B, and SCNN1G were 0.965, 0.979, and 0.988 based on the TCGA database (p?
Conclusion : The aberrantly decrease of SCNN1 family members might serve as valuable biomarkers for the diagnosis of ccRCC.
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KEYWORD
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Clear cell renal cell carcinoma, Diagnostic biomarker, Epithelial sodium channel, Hypermethylation
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